Ge affinity chromatography pdf

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Data file 18-1171-41 AB Affinity chromatography GE Healthcare Life Sciences Chelating Sepharose™ Fast Flow Chelating Sepharose Fast Flow is a BioProcess™ medium (resin) for immobilized metal ion affinity chromatography (IMAC) of native and histidine-tagged fusion proteins (Fig 1). The medium is suitable for both laboratory-scale purification and large-scale use, and is well-established in The default values represent the average chromatographic experiment and are used in many of the Standard Purification Protocols in this handbook. Creating a method can be achieved in a few simple steps: . Select a chromatographic technique 2. Select a suitable template. 3. Select an appropriate column from the Column list. 4. • Significant reduction in resin volume and increase in affinity chromatography productivity using 3C PCC (Table 1). • Product pool purity consistent across different feeds (Fig 4). GE, the GE Monogram, ÄKTA, Capto, HiPrep, Sephadex, UNICORN, and Xcellerex are trademarks of General Electric Company. Affinity Chromatography Principles and Methods 18-1022-29 Hydrophobic Interaction Chromatography Principles and Methods 18-1020-90 Gel Filtration Principles and Methods 18-1022-18 Reversed Phase Chromatography Principles and Methods 18-1134-16 Expanded Bed Adsorption Principles and Methods 18-1124-26 Chromatofocusing with Polybuffer and PBE 18-1009-07 Microcarrier cell culture Principles and Affinity chromatography has become the method of choice for the purification of monoclonal antibodies (Kelley, 2007, Kelley et al., 2009, Low et al., 2007), while the purification procedures for polyclonal plasma-derived antibodies traditionally incorporate precipitation steps combined with ion exchange chromatography. In this chapter we describe the potential advantages and draw-backs of Ion chromatography (or ion-exchange chromatography) is a chromatography process that separates ions and polar molecules based on their affinity to the ion exchanger. It works on almost any kind of High Performance. This prepacked column is ideal for preparative purification of Histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). The special design of the column, together with the high-performance matrix of the Ni Sepharose medium, provides fast, simple, and easy separations in a convenient format. GE Healthcare Bio-Sciences Corp., 100 Results Way, Marlborough, MA 01752 USA GE Healthcare Japan Corp., Sanken Bldg., 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073, Japan KA789220318HB 18102218 AM GE Healthcare Bio-Sciences AB Björkgatan 30 751 84 Uppsala Sweden Size Exclusion Chromatography Principles and Methods GE Healthcare Instructions 71-7086-00 AF Affinity media GE Healthcare CNBr-activated Sepharose™ 4B CNBr-activated Sepharose 4B is a pre-activated medium for immobilization of ligands containing primary amines. It provides a very convenient way to immobilize ligands by the cyanogen bromide method. The coupling reaction is spontaneous, rapid and easy to Affinity Chromatography. Affnity Chromatography Vol.2. 제품 선택 가이드 . Columns and resins for antibody purif