Gram staining lab report pdf

(PDF) Laboratory perspective of gram staining and its significance in investigations of infectious diseases Laboratory perspective of gram staining and its significance in investigations of After performing a gram stain, the technician should first determine whether the Gram stain is adequate. In an appropriately stained biological specimen, the nuclei of neutrophils are red. If the nuclei are blue, the decolorization is insufficient. Results Gram-negative bacteria will stain pink/red and Gram-positive bacteria will stain blue/purple. The counter stain used in the Gram stain is Safranin (pink). This is applied and stains the Gram negatives pink. To a Gram positive cell the counter stain stains the cell, but the cells are already so heavily stained by crystal violet that the addition of pink does not change the dark purple color of the cells. (aryal s., 2020) figure 1: the structure of cell wall of gram-positive and gram-negative bacteria there are four fundamental steps in gram staining, which include applying a primary stain of crystal violet to a heat fixed-smear of a bacterial culture, gram positive and gram negative will appear purple at this point due to the applied stain of … The procedure of the gram stain includes four different stains. The primary stain is the crystal violet and is poured onto the heat fixed smear left on for one minute and then gently rinsed off with water. The function of the primary stain is to stain the cell walls of all types of bacteria purple.