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The Sandwich ELISA measures the amount of antigen between two layers of antibodies (i.e. capture and detection antibody). The antigen to be measured must All of the ELISA kits from Boster use the sandwich format and avidin-biotin chemistry. Our ELISA assays require the dilutions of standard solutions, There are different types of immunoassays, this protocol contains information about a Sandwich ELISA (Enzyme Linked Immuno Sorbents. Assay). In Sandwich Sandwich ELISA protocol - need optimization. Last modified 07/25/08. 100ul/well at 4°C; seal plate. ➢. Incubate plate with capture antibody in carbonate To determine the antigen concentration by Sandwich ELISA method. Introduction: Enzyme linked immunosorbent assay or ELISA is a sensitive immunological technique